* M. Lima, * A.H. Ribeiro dos Santos, * L. Xavier , * A . Pinto Ribeiro, * J . Coutinho, ** A.L. Canelhas **F.A. Silvestre, **C. Peixoto, **C.Barbedo, ** J.M. Cabral, **S. David, **A. Paiva *B. Justiça.
*Service of Hematology and
Introduction: The investigation of patients with lymphoproliferative disorders (LPD) includes histological and immunophenotypic analysis of lymph node (LN) biopsies. This is usually performed by classic immunohistochemical (IH) methods, flow cytometric (FC) studies being rarely used in this case. The present study was aimed to evaluate the usefulness of routine immunophenotyping LN biopsies by FC in the diagnosis of patients with LPS.
Material and Methods: FC was performed on cell suspensions obtained from fresh LN from patients with clinically suspected or previously diagnosed lymphoma. The following monoclonal antibodies were used in the first approach, applying the following double stainings : CD14/CD45, CD2/CD19, CD8/CD4, HLA-Dr/CD3, þ /þ immunoglobulin light chains. Other B, T, NK or other cell markers were used when indicated.
Results : We present illustrative examples
of reactive and neoplastic LPD disorders whose LN were studied
by FC and classic IH. The results obtained by FC were
complementary to those obtained by IH. Reactive hyperplasias
had polimorphic and polyclonal lymphoid populations. B-cell
lymphomas usually showed a more monotonous population with an
aberrant monoclonal B-cell phenotype, although variable
proportions of reactive T cells were frequently found. T-cell
lymphomas were usually identified by an abnormal immature or
aberrant T-cell immunophenotype.
Comments