We have developed two mutants of S. cerevisiae affected in morphogenesis from parental strain D1 (A/þ, ura3/ura3, leu2/leu2, trpl, his1, his3). At 28ºC these mutants show higher size than parental strain. Budding formation process is different in both mutants producing aberrant buds localization. In order to study the distribution of actin filaments and actin total amount in these mutants we use FITC-Phaloidine (Sigma) in combination with flow cytometry and confocal microscopy.

By Flow Cytometry we have seen that 918 mutant has more actin than the other mutant and the parent strain, but its distribution is abnormal, as we can see by confocal microscopy. To study the phenomenon we developed actin overexpressing strain from 918 mutant to detect the accumulation site of the actin.

These mutants stop growing in specific cell cycle points, so they are called cdc mutants. To detect the exact moment where 918 and 327 mutants stop, we have developed several double mutants between our mutants and other cdc mutant studying the actin distribution during cell cycle. The results show that cdc mutants have affected morphogenesis pathways