Anti-Rat NKR-P1A, Clone 10/78

BACKGROUND

The 10/78 antibody reacts with NKR-P1A, a 60-kDa homodimer expressed on all natural killer (NK) cells and a small subset of T lymphocytes.1 The 10/78 antibody competes with the 3.2.3 antibody for binding to the antigen.1,2 NKR-P1A is a type II integral membrane protein with an extracellular C-type lectin domain, which is an NK cell-activating receptor specific for tumor target cells.3

USAGE

The antibody has been tested by immunofluorescent staining (lesser than or equal to 1 µg/106 cells) with flow cytometric analysis to assure specificity and reactivity. Other applications include immunoprecipitation.1 Since applications vary, each investigator must determine dilutions appropriate for individual use.

Cat. No. Description Clone Size
22641D Purified anti-rat NKR-P1A 10/78 0.5 mg
22642D Biotinylated anti-rat NKR-P1A 10/78 0.5 mg
22644D FITC labeled anti-rat NKR-P1A 10/78 0.5 mg
22645B PE labeled anti-rat NKR-P1A 10/78 0.2 mg

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES
1. Hünig, T. 1996. Personal communication.
2. Chambers, W.H., N.L. Vujanovic, A.B. DeLeo, M.W. Olszowy, R.B. Herberman, and J.C. Hiserodt. 1989. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. J. Exp. Med. 169: 1373 - 1389.
3. Ryan, J.C., E.C. Niemi, M.C. Nakamura, and W.E. Seaman. 1995. NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity. J. Exp. Med. 181: 1911 - 1915.

PharMingen also supplies a complete line of antibodies to rat cell surface antigens, including:

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu