SPECIFICITY

The OX-62 antibody reacts with an antigen on dendritic cells and possibly on T cells expressing gamma delta TCR.1 The antigen can be detected by flow cytometric analysis of dendritic cell-enriched leukocyte populations and by immunohistochemical staining of epidermal sheets and frozen sections of lymphoid and non-lymphoid organs.1,2 The OX-62 mAb immunoprecipitates molecules with integrin-like properties.1

USAGE

The antibody has been tested by immunofluorescent staining (lesser than or equal to 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections to assure specificity and reactivity. Other reported applications include immunoprecipitation and western blot analysis.1 Since applications vary, each investigator must determine dilutions appropriate for individual use.

Cat. No.	Description	Clone	Isotype	Size
22651D	Purified anti-rat dendritic cells	OX-62	Mouse IgG1	0.5mg

REFERENCES
1. Brenan, M., and M. Puklavec. 1992. The MRC OX-62 antigen: A useful marker in the purification of rat veiled cells with the biochemical properties of an integrin. J. Exp. Med. 175: 1457 - 1465.
2. PharMingen. Unpublished results.

Related products for rat immunology research include antibodies to CD11a, CD11b, CD11c, MHC Class II, and Mononuclear Phagocytes.

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu