PharMingen

Anti-Mouse CD1d, Clone 1B1
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DESCRIPTION

The 1B1 antibody reacts with mouse CD1d, a 48-kDa glycoprotein with structural homology to the major histocompatibility complex (MHC) class I molecules.1 Like MHC class I, CD1d associates with beta2-microglobulin, but similar to MHC class II, CD1d binds preferentially to long peptides with hydrophobic residues at specific positions.2 The mouse Cd1 gene family consists of two highly similar genes Cd1.1 and Cd1.2, which are homologous to human Cd1d.3 CD1d+ cortical thymocytes are postulated to mediate the positive selection of NK1+ T cells, which have a limited T-cell receptor repertoire and the exceptional ability to produce cytokines in response to primary stimulation.4,5 CD1d expression has been detected by mAb 1B1 at varying levels on most types of bone marrow and peripheral leukocytes and on epithelial, dendritic, and lymphoid cells in the thymus.1,6 The 1B1 antibody competes with mAb 1H1 (another antibody specific for mouse CD1d7) in binding to mouse splenocytes.6

USAGE

This antibody has been tested by immunofluorescent staining (lesser than or greater to 4 µg/ million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include immunohistochemical staining of acetone-fixed frozen sections.6 Since applications vary, each investigator must determine dilutions appropriate for individual use.

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES
1. Sydora, B.C., L. Brossay, A. Hagenbaugh, M. Kronenberg, and H. Cheroutre. 1996. TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes. J. Immunol. 156: 4209 - 4216.
2. Casta–o, A.R., S. Tangri, J.E.W. Miller, H.R. Holcombe, M.R. Jackson, W.D. Huse, M. Kronenberg, and P.A. Peterson. 1995. Peptide binding and presentation by mouse CD1. Science 269: 223 - 226.
3. Bendelac, A. 1995. CD1: Presenting unusual antigens to unusual T lymphocytes. Science 269: 185 - 186.
4 . Bendelac, A. 1995. Positive selection of mouse NK1+ T cells by CD1-expressing cortical thymocytes. J. Exp. Med. 182: 2091 - 2096.
5. Bendelac, A., O. Lantz, M.E. Quimby, J.W. Yewdell, J.R. Bennink, and R.R. Brutkiewicz. 1995. CD1 recognition by mouse NK1+ T lymphocytes. Science 268: 863 - 865.
6. PharMingen. Unpublished results.
7. Balk, S.P., S. Burke, J.E. Polischuk, M.E. Frantz, L. Yang, S. Porcelli, S.P. Colgan, and R.S. Blumberg. 1994. b2-microglobulin-independent MHC Class Ib molecule expressed by human intestinal epithelium. Science 265: 259 - 262.

Cat. No. Description Clone Size
09901D Purified anti-mouse CD1d 1B1 0.5 mg
09902D Biotinylated anti-mouse CD1d 1B1 0.5 mg
09904D FITC labeled anti-mouse CD1d 1B1 0.5 mg
09905A PE labeled anti-mouse CD1d 1B1 0.2 mg

PharMingen also supplies a complete line of antibodies to mouse cell surface antigens, including:

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu