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Click here to skip flow cytometry slides and go directly to confocal microscopy slides

Flow Cytometry - Theory and Application Slides

• Set 1:[Origin: Dr. Robert Murphy, Carnegie Melon University, Pittsburgh, PA]

  The basics of flow cytometry including fluidics, optics, electronics, sorting, and DNA and chromosome analysis.

  (file:murphy1.ppt, 52 slides) DOWNLOAD THE ORIGINAL PPT FILES

  Fluidics:

  (group 1) (group 2) (group 3) (group 4)

  Optics:

  (group 1) (group 2) (group 3) (group 4)

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  (file:murphy2.ppt, 10 slides) DOWNLOAD THE ORIGINAL PPT FILES

  Electronics and data aquisition:

  (group 1) (group 2)

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  (file:murphy3.ppt, 23 slides) DOWNLOAD THE ORIGINAL PPT FILES

  Sorting:

  (group 1) (group 2)

  DNA and chromosome analysis:

  (group 1) (group 2) (group 3)

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• Set 2:[ORIGIN: Purdue University Cytometry Laboratories]

  Basics of flow cytometry including:
  (file:flowtec.ppt, 17 slides) DOWNLOAD THE ORIGINAL PPT FILES

  Forward/Side Scatter, Filters,Optics

  Group 1

  Excitation and Emission Spectra for Commonly Used Dyes

  Click here to view sample slide or Group 2

  Explanations: teca.txt

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• Set 3:[ORIGIN: Dr. J.Paul Robinson, Purdue University Cytometry Laboratories]

  Applications of Flow Cytometry including:
  (file:floana.ppt, 14 slides) DOWNLOAD THE ORIGINAL PPT FILES

  Immunophenotyping, DNA Analysis, Oxidative Burst

  Click here to view sample slide or Group 1

  Chromosome analysis, Reticulocyte Analysis, and Calcium Influx

  Group 2

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• Set 4:[ORIGIN: Dr. Carleton Stewart, RPCI Laboratory of Flow Cytometry, Buffalo, NY]

  Tutorial on antibody binding and multiple color analysis

  (49 slides) (file:stewart.ppt) DOWNLOAD THE ORIGINAL PPT FILES

  Click here to view One sample slide or

  Group 1 Group 2 Group 3 Group 4

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• Set 5:[ORIGIN: Dr. J.Paul Robinson, Purdue University, IN]

  Slide set on DNA Analysis

  (16 slides) (file:dna1.ppt) DOWNLOAD THE ORIGINAL PPT FILES

  Group 1 Group 2 Group 3

  Slide set on DNA from Paraffin Blocks and Apoptosis

  (12 slides)(file:dna2.ppt) DOWNLOAD THE ORIGINAL PPT FILES

  Group 4 Group 4

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu