Table of Fluorochromes

David Chambers  http://flosun.salk.edu/~davidc

This is a table of characteristics of fluorochromes of use in flow cytometry. Within groups, roughly in order of excitation wavelength. Colors you might see with a capable browser are only a rough approximation :)

Probe Ex (nm) Em (nm) MW Notes
Reactive and conjugated probes
Hydroxycoumarin 325 386 331 Succinimidyl ester
Aminocoumarin 350 445 330 Succinimidyl ester
Methoxycoumarin 360 410 317 Succinimidyl ester
Cascade Blue 375;400 423 596 Hydrazide
Lucifer yellow 425 528    
NBD 466 539 294 NBD-X
R-Phycoerythrin 480;565 578 240,000  
PE-Cy5 conjugates 480;565;650 670   aka Cychrome, Tri-Color, Quantum Red
Red 613 480,565 613   PE-Texas Red
Red 670 480,565 670    
Fluorescein 495 519 389 FITC; pH sensitive
BODIPY-FL 503 512    
Cy3 512,552 565,615    
TRITC 547 572 444 TRITC
X-Rhodamine 570 576 548 XRITC
Lissamine Rhodamine B 570 590    
Texas Red 589 615 625 Sulfonyl chloride
Cy5 625-650 670    
Allophycocyanin 650 660 104,000  
Nucleic acid probes
Hoechst 33342 343 483    
DAPI 345 455    
Hoechst 33258 345 478    
Chromomycin A3 445 575    
Mithramycin 445 575    
Thiazole Orange 453 480    
YOYO-1 491 509    
Ethidium Bromide 493 620    
Acridine Orange 503 530/640   DNA/RNA
TOTO-1, TO-PRO-1 509 533   Vital stain
Propidium Iodide (PI) 536 617 668  
TOTO-3, TO-PRO-3 642 661    
Cell function probes
Indo-1 361/330 490/405 1010 AM ester. Low/High Ca2+
Fluo-3 506 526 855 AM ester. pH > 6
2'7'Dichorodihydrofluorescein (DCFH) 505 535 529 Oxidized form
Dihydrorhodamine 123 (DHR) 505 534 346 Oxidized form. Light catalyzes oxidation
SNARF 548/579 587/635   pH 6/9
Green Fluorescent Proteins
Y66F 360 508    
Y66H 360 442    
Y66W 436 485    
Wild Type 396,475 508,503    
S65A 471 504    
S65C 479 507    
S65L 484 510    
S65T 488 511    
Other probes
Monochlorobimane 380 461 226 Glutathione probe
Calcein 496 517 623 pH > 5

Legend:

Back to teaching materials

Back to handy tools

Home Page Table of Contents Sponsors E-Mail Archive Web Sites
Source of this page: Salk Flow Cytometry

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu