Monoclonal Antibodies and Conjugates to Mouse Leukocyte Markers

Product Number Monoclonal Antibody to: Label Rat Ig
Class 		Clone
F 7275 Mouse CD3 FITC IgG2b 29B
P 2817 Mouse CD3 PE IgG2b 29B
R 3512 Mouse CD3 Quantum RedTM IgG2b 29B
F 7400 Mouse CD4 FITC IgG2a H129.19
P 2942 Mouse CD4 PE IgG2a H129.19
R 3637 Mouse CD4 Quantum RedTM IgG2a H129.19
F 6027 Mouse CD5 (Ly-1) FITC IgG2a 53-7.3
P 3444 Mouse CD5 (Ly-1) PE IgG2a 53-7.3
R 8138 Mouse CD5 (Ly-1) Quantum RedTM IgG2a 53-7.3
F 7525 Mouse CD8a FITC IgG2a 53-6.7
P 3067 Mouse CD8a PE IgG2a 53-6.7
R 3762 Mouse CD8a Quantum RedTM IgG2a 53-6.7
F 3152 Mouse CD11a
(LFA-1a) 		FITC IgG2a I21/7
P 6693 Mouse CD11a
(LFA-1a) 		PE IgG2a I21/7
R 5388 Mouse CD11a
(LFA-1a) 		Quantum RedTM IgG2a I21/7
F 3027 Mouse CD18
(LFA-1b) 		FITC IgG2a C71/16
P 6818 Mouse CD18
(LFA-1b) 		PE IgG2a C71/16
R 5513 Mouse CD18
(LFA-1b) 		Quantum RedTM IgG2a C71/16
F 7775 Mouse CD25
(IL-2 Receptor) 		FITC IgG2a AMT-13
P 3317 Mouse CD25
(IL-2 Receptor) 		PE IgG2a AMT-13
R 4012 Mouse CD25
(IL-2 Receptor) 		Quantum RedTM IgG2a AMT-13
F 7900 Mouse CD45 FITC IgG2a I 3/2
P 3442 Mouse CD45 PE IgG2a I 3/2
R 4137 Mouse CD45 Quantum RedTM IgG2a I 3/2
F 8025 Mouse CD45R FITC IgG2a RA3-6B2
P 3567 Mouse CD45R PE IgG2a RA3-6B2
R 4262 Mouse CD45R Quantum RedTM IgG2a RA3-6B2
F 2527 Mouse CD45R-1 FITC IgG2a 16A
P 6193 Mouse CD45R-1 PE IgG2a 16A
R 4888 Mouse CD45R-1 Quantum RedTM IgG2a 16A
F 2652 Mouse CD71
(Transferrin receptor) 		FITC IgG2a RI7217
P 6318 Mouse CD71
(Transferrin receptor) 		PE IgG2a RI7217
R 5013 Mouse CD71
(Transferrin receptor) 		Quantum RedTM IgG2a RI7217
F 2902 Ia FITC IgG1(mo) OX-6
P 6568 Ia PE IgG1(mo) OX-6
R 5263 Ia Quantum RedTM IgG1(mo) OX-6
F 3277 Mouse Pgp-1
(Ly 24) 		FITC IgG2a IM7.8.1
F 2277 Mouse TCR A/B FITC IgG
(hamster) 		H57-597
P 5943 Mouse TCR A/B PE IgG
(hamster) 		H57-597
R 4638 Mouse TCR A/B Quantum RedTM IgG
(hamster) 		H57-597
F 3402 Mouse ThB FITC IgG2a RGRSL114.8.1
P 7068 Mouse ThB PE IgG2a RGRSL114.8.1
R 5763 Mouse ThB Quantum RedTM IgG2a RGRSL114.8.1
M 7898 Mouse Thy 1.1
(fractionated mouse ascites fluid) 		IgM
(mo) 		TN-26
F 2887 Mouse Thy 1.1 FITC IgM
(mo) 		TN-26
F 7650 Mouse Thy 1.2 FITC IgG2b 30-H12
P 3192 Mouse Thy 1.2 PE IgG2b 30-H12
R 3887 Mouse Thy 1.2 Quantum RedTM IgG2b 30-H12
M 8023 Mouse Thy 1.2
(fractionated mouse ascites fluid) 		IgM
(mo) 		TS
F 3012 Mouse Thy 1.2 FITC IgM
(mo) 		TS
M 8148 Mouse Thy 1.2
(in vivo cytotoxic) 		IgG2a
(mo) 		TC
Sigma Monoclonal Antibodies to Mouse Leukocyte Markers (CD Antigens) and their conjugates are evaluated for their performance by flow cytometry and formulated to obtain maximum percent positive staining without significant elevation of background staining. Each vial contains 100 mg in approximately 0.4 ml (approximately 0.25 mg/ml), 1 mg of conjugate is usually sufficient to stain 1 x 106 cells to maximum fluorescent intensity and percent positive.

Sigma FITC conjugated mouse CD antibodies are prepared by conjugation of fluorescein isothiocyanate isomer I (FITC) with the purified rat immunoglobulin fraction from ascites fluid. The products are supplied in 0.01 M phosphate buffered saline, pH 7.2, containing 2 mM EDTA, 1% BSA and 0.1% sodium azide as preservative.

Sigma R-Phycoerythrin (PE) conjugated mouse CD antibodies are prepared by conjugation of R-phycoerythrin with the purified rat immunoglobulin fraction from ascites fluid according to a modification of the method of Kronick.(1) The products are supplied in 0.01 M phosphate buffered saline, pH 7.2, containing 2 mM EDTA, 1% BSA and 0.1% sodium azide as preservative.

Quantum RedTM is a tandem fluorescent dye created by coupling R-phycoerythrin with Cy5 reactive dye. This dye is efficiently excited at 488 nm and emits at 670 nm. It is useful as a third color in combination with fluorescein and R-phycoerythrin for multiparameter analysis. It is useful as a second color in combination with fluorescein when no compensation requirement between the green and red photomultiplier tubes is desired. Quantum Red is also useful as the single color when there is high autofluorescence of the cells. Sigma Quantum Red conjugated mouse CD antibodies are prepared by conjugation of Quantum Red to purified rat immunoglobulin fraction from ascites fluid according to a modification of the method of Kronick.(1) The products are supplied in 0.01 M phosphate buffered saline, pH 7.2containing 2 mM EDTA, 5% glycerol, 1% BSA and 0.1% sodium azide as preservative.

Reference:

1.Kronick, M.N., J. Immunol. Meth., 92, 1 (1986).

Store at 2-8°C.Shipping Conditions: Ambient.

U.S. Patent No. 4,859,582; 4,542,104

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu