I=B4m interested in detection/quantification of cytoplasmatic and/or
intranuclear STAT proteins by fluorescence microscopy or flow.
Has anybody experience with these proteins? E.g.:
- Is the amount of protein/cell above the detection limit?
- Are fixation and staining protocol(s) available?
All papers I found so far describe use of anti-STAT mAbs for Western
blotting analysis etc. but not for immunofluoescence stainings.
Every suggestion is welcome!
Thanks in advance
Georg
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H.G. Kreysch
Merck KGaA
Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323=20
Frankfurter Str. 250 FAX: (49)6151 - 72 7398
D-64271 Darmstadt E-Mail: kreysch@merck.de