What volume of bone marrow do you aspirate for flow. If you aspirate too
much you get blood, not bone marrow. However, you want as much as possible
from a single site. I had thought 1mL per site was maximum but was told
today that 3 mL gives a good yield. Please share you specifications and I
will summarize for those interested.
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH