RE: A clean machine -Reply

Thomas Delohery (t-delohery@ski.mskcc.org)
Mon, 17 Feb 1997 12:02:24 +0530

Seems like we're the only ones that use detergent. We fill the tubing
with a 60cc syringe with 1:100 7X detergent in millipore H2O and let it
sit for an hour or two. This is followed by a millipore H2O rinse, then
60cc of 70% EtOH to help flush the detergent, followed by half a liter of
PBS. The 7X and EtOH is filtered with a 0.45 micron syringe filter.
Detergent is never used in the sheath tank or filter housing. We do this
about once per month and it works beautifully.

The only time I use bleach is if there's a known contaminant and we haven't
had one of those in many years. Bleach does some pretty horrible things to
the tubing not to mention your nozzle tip.

good luck,
td

--
==============================================================================
 Thomas Delohery                        | Internet: t-delohery@ski.mskcc.org
 Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729
 Memorial Sloan-Kettering Cancer Center |      Fax: (212) 794-4019
 1275 York Ave. Box 98                  |
 New York, NY    10021                  |
==============================================================================