Re: BD fastimmune system

Michelle N Fiordalisi (mnfiord@med.unc.edu)
Fri, 21 Feb 1997 08:44:21 -0500 (EST)

I have been working with BDs FastImmune anti-IFNg and anti-IL-4
antibodies for about 2 months (but only with normal controls) and have
been very pleased with the results. I have been following BD's protocol
and have had success with each experiment. I have used both the
IFNg/IL-4 combo with the isotype combo and individual reagents with
success. Although BD sells a special formulation of IgG1 PE, I have been
titering their standard formulation and think it will work nearly as well
as a control for IL-4 PE.

I have not tried using saponin for permeablization but will be comparing
BD's FACSPerm to Caltag's Fix N' Perm.

If you would like more details, please email me directly.

-- Michelle
===========================================================================
Michelle N. Fiordalisi, Ph.D.
Clinical Immunology Fellow
University of North Carolina Hospitals
email: mnfiord@med.unc.edu
Phone: 919-966-4058 FAX: 919-966-0486
===========================================================================

On Thu, 20 Feb 1997, Vishal Gulati wrote:

>
>
> Hi! Has anyone on the list used BD Fastimmune Intracellular cytokine
> staining reagents. I have been using 'conventional' indirectly lablled
> cytokine antibodies and am planning to switch over.
>
> Also, has someone compared saponin with BD/any other commercial
> permeablising reagent .
>
> Id be grateful if those of you out here in the know of things share their
> experiences.
>
> Thanks in advance
>
>
> Bye
>
>
> Vishal
>