David McFarland
University of Illinois Biotechnology Center
Flow Cytometry Facility
---------- Forwarded message ----------
Date: Wed, 26 Feb 1997 13:35:59 +0000
From: tlr@mrc-lmb.cam.ac.uk
To: cyto-inbox
Subject: PHA aggregation
Dear colleagues,
I am looking at intracellular cytokines produced by CD14+/CD3+ cells from
PBMC cultures, in response to PHA stimulation. I am having problems with
cell aggregation . Has anyone used a similar system and how did they get
round it? Stimulating with PMA/ionophore is not an option at the present
time.
Thank you in advance,
Tom Rowland