Exporting figures from cell quest: how?

Murali Ramanathan (murali@ACSU.Buffalo.EDU)
Tue, 25 Mar 1997 21:25:07 -0500

Hello everybody

I have had the same problem. If you want classy
graphics you have several options and none of
them are simple.

There are several freeware/shareware programs
that can read files created by Cell Quest.

FCS Assistant ($25 shareware for the Macintosh) can
read these files and convert them to ASCII.
You can then plot the data in the graphing program of
choice. I am not sure whether FCS assistant allows
you to save gated files. I havent used it much.

If you have a Windows machine, get WinMDI which
lets you save histograms in ASCII. It also can save
gated histograms and dot plots.

Both FCS Assistant and WinMDI can be accessed from
the U Mass flow cytometry software page.
http://www.bio.umass.edu/mcbfacs/flowcat.html

Another approach is to scan the plots
of interest and then use DataThief (Available from the
NIH Image site, I dont know the URL) to "steal" the
x-y coordinates. This is really useful if you dont
have acess to the original files and are stuck with
a lousy print out. Data Thief creates a text file that
can be read in any graphing program. This does not
work for dot plots.

Hope that helps

Regards

Murali Ramanathan