STAINING FIXED PLATELETS

gumbsc@CENTOCOR.COM
Tue, 28 May 1996 11:17:44 -0400

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Has anyone had success in staining fixed platelets with a p-selectin marker (eg. S12-FITC
or CD62)? Becton Dickinson claims that it can be done using 1% paraformaldehyde
fixation. I have followed their protocol for activation (substituting PMA for ADP) and
fixation. I have been able to stain fixed platelets with a pan-platelet marker (10E5 and
CD42a) but not the p-selectin markers. (All markers perform successfully on unfixed
platelets.) Any insights?

Cindy Gumbs
Assoc. Scientist II
Centocor, Inc.
200 Great Valley Pkwy
Malvern, PA 19380
(610) 696-7668
FAX (610) 651-6201

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