re:re: intracellular IL4 and IFNg

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Fri, 31 May 96 18:00:50 +0200

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We have done analysis in mouse lymph node T cells=20
after in vitro culture. After 4 days or more of=20
culture w ConA and then a 6 hour PMA/ionomycin=20
treatment in the presence of monensin we see=20
abundant IFN gamma and some IL4. They are not=20
expressed in the same cells.

We use 50 ng/ml ConA and 50 ng/ml PMA and 1 ug/ml=20
ionomycin in the presence of 3uM Monensin.

Mikael

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