Re: fixing and storing cells
Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Thu, 13 Jun 1996 12:36:50 +1100
At 09:03 PM 11/06/96 -0700, Ruedi Braun wrote:
>
>Does anyone have experience with the fixation of cells after staining
>with FITC or PE?
>I am using 1% paraformaldehyde in PBS and analyze the cells within 12
>hours after fixation.
>We are planing to do some experiments with lavages on humans at high
>altitude and I won't be able to get to a FACS for at least 5 days. Is
>there a possiblity to fix stained cells for such a long time?
It should work, you will probably see an increase in background, but
that should still be usable provided your staining is bright enough to
separate pos. from neg.
I would try out all the steps, including delays, on the ground first to
see what happens in your own hands.
Good luck, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-565-6110 Fax: 61-2-565-6101