RE: fixing without leakage

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Mon, 17 Jun 1996 08:43:25 -0400

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My impression is that paraformaldehyde fixation does not permeabilize or
at least does not permeaboilize efficiently. I guess the real test would
be to try to stain for an intracellular protein, say actin, without
permeabilization.

Calman Prussin

>----------
>From: Deborah Berglund
>Sent: Friday, June 14, 1996 2:26PM
>Cc: Cytometry Mailing List
>Subject: Re: fixing without leakage
>
>
>Hi everyone,
>
>We want to be able to fix (stop metabolic action, etc.) some cells in a
>
>monolayer. All of the fixatives I am familiar with permeabilize the
>cells and we can't have that! Any ideas?
>
>Going into the mountains tomorrow with llamas carrying all my gear!
>Luxury or lazy?
>
>Deb Berglund
>Montana State University
>Bozeman, MT 59715
>
>Montana, no longer in the nightly news due to FREEMEN
>
>

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