Mouse flow

Jeff Carrell (Jeff_Carrell@hgsi.com)
Mon, 01 Jul 96 14:06:22 EST

If anyone routinely does flow on peripheral blood from mice and could offer
advice, hints or tricks, I could really use the help.

We are using a BD FACScan. We are currently using Gentrak lyse/fix, although I
think we're going to try the BD stuff.

I'm looking simply at what changes occur in the basic lineages (mono, gran,
lymph) when the mice are treated with novel proteins. I am using a three tube
panel: isotype control, B220/CD3 and CD14/Gr.1 (two color).

I'm more familiar with human LWB; the mouse FSC v. SSC looks similar but the
monocytes aren't as clearly separated--is this usually the case with mice?

Any mouse flow hints would be great,

Thanks,
Jeff Carrell
e-mail:
jeff_carrell@hgsi.com