We wish to determine ploidy of industrial yeast (up to 6n) from
listmode FACScan data. We are using 7-AAD at low concentration so
aren't too worried about rRNA. We grow the cells to minimise
mitochondrial interference. We therefore only need a program to take
account of: cell cycle stage/cells with attached buds/doublets.
Are you familiar with a good program for this that we can use for free (or
cheap!)
Thanks for your help,
Dan
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Dr Dan Deere (ddeere@rna.bio.mq.edu.au)
Research Fellow
Australian Environmental Flow Cytometry Group
School of Biological Sciences,
Macquarie University,
Sydney,
Australia NSW 2109.
Tel- +61 2 850 8150/8238 or 8685510
Fax- +61 2 850 8245
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