Dr. Sharp,
Though I believe it is possible to obtain good chromosome
resolution when exciting with the 458 nm laser first, I would not
recommended it.
I prefer triggering on UV excited fluorescence since it is brighter
(usually 2-4X) and exhibits better chromosome peak resolution than
fluorescence that results from excitation at 458 nm.
=====================================================================
John J. Fawcett, fawcett@lanl.gov
Life Sciences Division, LS-3 505/667-9633 : office(vmail)
Los Alamos National Laboratory 505/667-2750 : LS Business Unit
P.O. Box 1663, ms M888 505/665-3024 : FAX
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