RE: Antibody conjugation
H.-G. Kreysch (kreysch@merck.de)
Wed, 07 Aug 1996 14:47:14 +0200
In our lab different murine (and one humanized) monoclonal antibodies were
labelled using FITC "isomer I" (e.g. Molecular Probes F-1906) with good
results (protocol available on request). Aliquots of labelled antibodies are
stored at -70=B0C and - in our experience - stable for many years.
In one case binding properties of an antibody were lost due to the
conjugation procedure.
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H.G. Kreysch
Merck KGaA
Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323=20
Frankfurter Str. 250 FAX: (49)6151 - 78 1332
D-64271 Darmstadt E-Mail: kreysch@merck.de