Re: PI disposal

N.W. BLACKHALL (PLZNB@pln1.life.nottingham.ac.uk)
Sun, 18 Aug 1996 10:58:59 GMT0BST

Dear Colleagues,

Dean Hewish recommends adding sodium hypochlorite to ethidium bromide
solutions. However, reference to Sambrook Fritch and Maniatis
(Molecular Cloning, a lab manual) turned up the following:

"Treatment of dilute solutions of ethidium bromide with hypochlorite
is not recommended as a method of decontamination. Such treatment
.... converts the dye into a compound that is mutagenic ....
Quillardet P and Hofnung M Trends Genetics 4:89 (1988).

Draw your own conclusions,

Nigel Blackhall
University of Nottingham.