Lysis Recipe
JOY MUNDY (jmundy@immuno.imvs.sa.gov.au)
Thu, 29 Aug 1996 15:21:47 GMT+1030
Can anybody help with a recipe for a RBC lysis solution that I can
use in conjunction with surface Kappa and Lambda staining. We want to
lyse, wash, stain and then fix cells. Currently we use ammonium
chloride with mixed sucess, we lose scatter sometimes and sometimes
the RBC take an age to lyse. We use 2ml lysis per 100ul cells.
Joy Mundy
Dept. Human Immunolgy
Institute of Medical & Veterinary Science
Adelaide, South Australia 5000
Ph 61-8-2287476; Fax 61-8-2324092
jmundy@immuno.imvs.sa.gov.au