Thus, I would like to solicit ideas and examples from the community. There are
the major things, like bad compensation (and what that leads to); more subtle
problems like mistaken inferences from viewing dot plots instead of contour
plots; and related topics like the problems of Cy5 "binding" to monocytes.
I.e., I'm looking for things that a person who is just trying to do a simple
experiment might encounter but wouldn't realize without the years of experience
that an operator might have. General topics might include: sample preparation
(this should only include facets specific to flow cytometry); instrumentation;
data analysis; and data presentation.
Please respond directly to me: Roederer@Darwin.Stanford.Edu. Please send
things you think are obvious (I rarely think of obvious things!), send me your
pet peeves, etc. Unless you specify, anything that results will acknowledge
your contribution.
mr