PREAMBLE:
In the wavelength continuum for excitation of fluorochromes, 488nm is the
Everest.
In the quest for extra colours, we can add tandems for 488-excitation, or
we can add light sources which move to higher or to lower wavelengths of
excitation. But which way is better?
Is the He-Ne path of higher wavelength excitation superior to the He-Cd (or
Hg arc) path of shorter wavelength excitation?
The weight of activity definitely vindicates 488 tandems and He-Ne, but why
not UV conjugates like AMCA and tandems for them? Why not viloet-excited
conjugates? Was not the choice driven by what was easy at that time, and
should we not now review it?
THE QUESTION I AM PUTTING: IS CONJUGATE-SPACE SYMMETRIC, OR IS IT BETTER
RED THAN DEAD-violet,..-indigo,...blue??
Have we given UV and violet a fair go in the antibody-fluorochrome
conjugate challenge?
What are the fluorochromes which are suitable conjugates for monoclonal
antibodies, and which excite in the violet or the UV?
Can you give me your tuppence-worth on this?
....still wondering,... Bob