Dear Dr. Wang,
The problem you are having labeling with pulsed BrdU and surface markers at
the same time is not unique to your laboratory. However, there is a
solution. I refer you a new methodology called SBIP ( Strand Break Induced
Photolysis). The methodology is outlined in the paper by Li, X.
Darzynkiewicz, Z: Labeling DNA strand breaks with BrdUTP. Detection of
apoptosis and cell proliferion, Cell Proliferation, 28, pp571-579, 1995.
Phoenix Flow Systems will be releasing a kit based on this methodolgy
August 15.
Regards,
C. Kevin Becker
Phoenix Flow Systems, Inc.
11575 Sorrento Valley Rd. #208
San Diego. CA, 92121 USA
(619) 453-5095 Fax (619) 259-5268
info@phnxflow.com
> From: Xiao-Tian Wang (Medical Education) <wangx01@doc.mssm.edu>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Question about Brdu double staining
> Date: Wednesday, July 02, 1997 5:46 PM
>
>
>
> Dear collegues:
>
> In my laboratory work, I attempt to double stain the cells with Brdu (for
> proliferation assay) and a cell surface marker. The cells I am working
> with are the cell mixture of intestine epithelial cells and T cells. I
> don't know if anybody has tried to do the similar things. It seems a
> little hard to do since the anti-Brdu Ab has to enter the nucleus while
> the other Ab has to bind to the cell surface moleculels. I would
> appreciate any input. My email address is wangx01@doc.mssm.edu
>
> Tian Wang
> Clinical Immunology
> Mt. Sinai School of Medicine
> Tel:(212)-824-7466
>