------------------------ Forwarded Message Follows - - - - - - -
Hi-
I hope this is the proper route to post something on the archive. I've
tracked down several protocols for propidium staining, but I do have one
question they do not address. Don't you need to centrifuge the stained
samples before running them through the flow cytometer? I would
imagine that you still have some PI in solution, and that it would give
you an awfully high background signal. Any comments?
Thanks. This is a wonderful forum.
Bob Crow
crow@cbnmr.tamu.edu
----------------------------------
posted by
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517
web http://www.cyto.purdue.edu