PI Staining

Gary Durack (durack@ux1.cso.uiuc.edu)
Wed, 9 Jul 1997 15:16:37 -0500 (CDT)

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It is usually not necessary to wash out PI because bound PI has a much
higher quantum efficiencey than free dye. Also, most flow cytometer
electronics are designed to ignore constant, or DC background such as a
fixed concentration of free dye in solution.

gary

Gary Durack
University of Illinois Biotechnology Center
voice (217) 244-0559 fax (217) 244-0466
durack@uiuc.edu
http://www.life.uiuc.edu/biotech

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