Rat CD4+OX22+ lymphocytes

RALPH SMIALOWICZ (SMIALOWICZ.RALPH@EPAMAIL.EPA.GOV)
Fri, 11 Jul 1997 08:42:09 -0400

Has anyone quantified CD4+OX22+ rat splenic lymphocytes that have not
been enriched? We want to quantify this subset, but have been
unsuccessful in attempts to discriminate W3/25+ OX22+ cells from whole
spleen preps. We are aware of several publications in which
enrichment was employed to characterize this subset. However, is
enrichment (i.e., either positive or negative) necessary? Would
appreciate any information on this topic.

Thanks,
Ralph Smialowicz
U.S. EPA
RTP,NC