Re: Three Colour Compensation

Fumio Ike (fike@rkna50.riken.go.jp)
Fri, 2 Oct 92 20:17:17 JST

In message <01GPCOOHQUV28Y4WQN@Darwin.Stanford.EDU> you write
>
> However, if the Facscan in question is not used for PI DNA analysis on FL3,
> a partial solution would be to replace the FL3 filter (a 650 LP) with one
> better optimized for the Cy5-PE spectrum. On our Facstar we use a 685 BP
> filter (which is centered around the Cy5-PE peak). This would minimizes the
> FL2 to FL3 compensation thus reducing the FITC overcompensation in FL3.
> This filter also allows enough PI signal to get though if it is used for
> live/dead cell gating; but not enough for good quality DNA analysis.
>

I am also troubled in three colour compensation problem on FACSort.

Could you give me more detailed information on the 685nm BP filter?
For example: 685nm +/- 30nm and laser beam quality(?)

Thanks for any information

Fumio Ike, PhD. E-mail:fike@rkna50.riken.go.jp
Animal and Cellular Systems Laboratory TEL:+81-48-462-1111(ext5042)
The Institute of Physycal and Chemical Research (RIKEN) FAX:+81-48-462-4681