frozen lymphocytes

auger@ux1.cso.uiuc.edu
Wed, 24 Feb 93 15:38:53 CST

Can anyone give me references to working with cell surface antigens (i.e.
CD4/CD8) on frozen/thawed lymphocytes? Is it possible without introducing
artifact? What kind of conditions (freezing/thawing) must be met to make
the procedure possible?

I imagine that there have been some published reports on the feasibility
of freezing isolated lymphocytes and then staining them with various
monoclonals, but quite frankly, if someone can send me their advice, it
saves me from going to the library!

I would appreciate any and all comments. Thanks.
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Julie A. Auger, Director 217-244-0559 voice
Flow Cytometry Facility 217-244-0466 fax
University of Illinois at Urbana-Champaign auger@ux1.cso.uiuc.edu
231 PABL, 1201 W. Gregory, Urbana, IL 61801
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