A FACS related problem

sanjeev@nii.ernet.in
Fri, 24 Sep 93 20:16:40 +0530

A certain molecule is expressed on the surface of two different cell lines at
similar levels, but (probably) in two different conformations. I have a
monoclonal that (probably!) discriminates between the two conformations. In
comparing the staining pattern of these two cell lines I face the following
problem.
The (no first Ab) conjugate controls for these two cell lines stain at two
different levels, so that it becomes impossible to compare the test
stainings.
1)If I change the voltage for one cell line to match its control staining to
that of the other, won't it bring about a more dramatic (and unreal) effect
on the test staining for this particular cell line?
2)Or can I just superimpose the two control stainings and compare then compare
the stainings with the monoclonal in question?
If anyone could throw some light on this aspect of FACS data analysis I will
be really greatful.
Pl. send your replies at the following address:

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SANJEEV KUMAR TEL>91-11-6863004
IMMUNOBIOLOGY LABS FAX>91-11-6862125
NATIONAL INSTITUTE OF IMMUNOLOGY TELEX>031-66183 NII IN
ARUNA ASAF ALI MARG GRAMS>IMMUNOLOGY
NEW DELHI-67 EMAIL> Sanjeev@NII.ernet.in

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