RE: NON-HUMAN PRIMATE FLOW CYTOMETRY

salmonm (salmonm@rheuma.bham.ac.uk)
Tue, 5 Oct 1993 10:20:34 +0100 (CET)

> In response to your question concerning staining of non-human primates for
> flow cytometery, Anne Brodie from the Yerkes Regional Primate Center in
> Atlanta, Georgia has informed me that the BD reagents Leu3a and Leu2a
> (anti CD4 and anti-CD8 respectively) work very well for the Great Apes as
> well as Pigtail and Stumptail macaques. However, their Leukogate product
> does not work very well. She has informed me that there might be some
> gating tricks in order to obtain optimal staining. Please give her a call
> at (404)727-7757 for further information.
>
> Good luck,
> Thomas Rowe, Centers for Disease Control and Prevention, Retrovirus
> Diseases Branch
> Atlanta, Georgia

I must have missed this earlier. If it is any use, the antibodies available
from Coulter: anti-2H4 (CD45RA) and 4B4 (CD29) which identify stages of T
cell differentiation, stand a very strong chance of working in non-human
primates, because they were both originally produced by immunization of mice
with cells from a non-human primate, namely Aotus trivirgatus, the owl
monkey

Have fun

Mike Salmon

E-mail: salmonm@rheuma.bham.ac.uk
Tel: 44 (0)21 414 6780
FAX: 44 (0)21 414 6794