Re: Quenching of fluorescein

Dave Coder (dave@nucleus.immunol.washington.edu)
Wed, 20 Oct 93 08:28:32 -0700

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One way has been to resuspend cells exposed to bacteria in a solution that contains
crystal violet which will quench fluorescein; ingested fluorescein-labeled bacteria
will stil fluoresce. Citations are in older literature 1982-1984. I can dig out some
if anyone is interested

Dave Coder
dcoder@u.washington.edu

Begin forwarded message:

Message-ID: <"70906191013991/3693 04061450*"@MHS>
To: list:;
Subject: Quenching of fluorescein

We want to measure phagocytosis of fluorescein-labeled bacter-
ia. To discriminate adhering from ingested particles we would

like to quench fluorescence of extracellular bacteria.

Is there any easy means besides use of a quenching anti-flou-
rescein antibody. A protocol or relevant citation of literature

would be gratefully acknowledged.

Please send response by E-mail. Thanks a lot.

Greetings from Frankfurt, Jo Betz.

------------------------------ End of forwarded message 1

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