In my limited analysis on a Coulter XL and an Elite, distilled water gives a
different FSvsSS pattern with less separation of monos from lymphs
(everything falls down and to the left) in Q-prepped blood samples. I
hypothesized at the time that some sort of hypotonic/hypertonic gig was
going on at the sheath/sample interface...so I stuck with the sheath fluid.
Ray Biagini
Research Toxiciologist
CDC/NIOSH
DBBS, ABB, IRS
Robert A. Taft Laboratories
4676 Columbia Parkway
Cincinnati, OH 45226
REB4@NIOBBS1.EM.CDC.GOV
Voice (513)533-8196
Paperless Fax (513)533-8400
Fax (513) 533-8510
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/