dye soln. and blank beads as a standard

vanburen%flovax.dnet@rocdec.roc.wayne.edu
Fri, 19 Nov 93 15:28:30 -0500

We have recently started work on an application in need of standards. We
would like a UV-excitable (350-365nm from Ar+ laser), violet-fluorescing
(400-450nm) standard. Most beads are either too bright (FCSC Hoechst, Indo,
and FullSpectrum) or too dim (FCSC Blank). I remember hearing about a
method using a dye solution with blank beads as a trigger source. Does
anyone have a procedure worked up for this method, or remember a source for
one? Any other ideas?

/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Immunology & Microbiology
\_^_/ Wayne State University