emits in the high 400's (465-490)."
Jeff Wang makes excellent beads for this purpose. His RFP-30-5A product is a
single bead that has fluorochromes that excite in uv and fluoresce into violet
to blue (ca. 430 to 490nm); they also excite at 488nm and fluoresce from the
green to the red (530 to 680nm). We use them for instrument alignment when
doing 5 color. The intensity of this single bead is typical of many labeled
cells.
There is also a mix of beads (RCP-30-5) that is comprised of 6 populations with
vary amounts of the fluorochromes. This provides a blank and 5 labeled beads
that span the entire histogram for green to red emissions, and about half the
half the histogram for blue and violet.
The forward scatter cv's are about 1.5% and linear green fluorescences are
mostly below 2% as I recall. Particle stability is excellent: at least 3 years
would be a good guess if refrigerated.
Jeff Wang is at:
Spherotech, Inc.
1840 Industrial Dr. Suite 270
Libertyville IL 60048
tel. 708-680-8922
fax. 708-680-8927
Dave