To the list:
To add to the information already provided, PharMingen now offers a few
monoclonals to swine leukocytes:
anti-CD1 clone 76-7-4
anti-CD2a clone 1038h-8-31
anti-CD4a clone 74-12-4
anti-CD8a clone 76-2-11
anti-SWC3a (monocytes/granulocytes) clone 74-22-15A
anti-pan pig tissue clone 1030h-1-19
anti-SLA-DR clone 1053h2-18-1
These are purified immunoglobulins from tissue culture. Most of them are from
the Second Swine CD Workshop (Immunology Today 17: 352-354, 1996), and some of
them are the same clones as those offered by VMRD (as clarified, filtered
ascites). We hope to expand our offering of antibodies to swine leukocytes in
the months to come, so please inquire about specificities which do not appear
on this list. If you need any further information, please contact
PharMingen's technical service department at techserv@pharmingen.com or phone
800-825-5832 in the US and Canada.
Florence Harrod
Product Manager, Research Immunocytometry
PharMingen
San Diego, CA, USA
--------------------------------------
Date: 9/23/97 1:24 PM
To: cyto-inbox
From: Gerhard Nebe-von-Caron
If you do a literature search on the subject you might find
a useful address in Tuebingen, Germany.
SUMMARY OF WORKSHOP FINDINGS FOR PORCINE T-LYMPHOCYTE
ANTIGENS
SAALMULLER A, AASTED B, CANALS A, DOMINGUEZ J, GOLDMAN T,
LUNNEY JK, MAURER S, PESCOVITZ MD, POSPISIL R, SALMON H,
TLASKALOVA H, VALPOTIC I, VIZCAINO JS, WEILAND E, ZUCKERMANN
F
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
43: (1-3) 219-228 OCT 1994
Dr. Armin Sallmueller is a very helpful person. He actually
laid out a 6 tube set of for a very comprehensive analysis
to me using supernatants and isotype specific multicolour
second step antibodies from Southern Biotecnology.
Unfortunately the group that had initiated the work went for
a set of FITC labeled antibodies from VRMD which gave far
less information.
Be aware of the CD45 from VRMD to be an IgM. I did not know
until we had finished our study with 'interresting' CD45
results.
Serotec offers an cd45 IgG2a and a whole range of others
including a cd25.
The cross reactivity patterns of anti human CD14 have not
been consistent. Some antibodies only worked with older
pigs. As their immunesystem is changing from NK to T-cell
response within the first 3 to 6 month you might want to use
older ones anyhow - which makes the sampling much more
interesting (don't forget to take a camera).
Contact addresses:
Dr. A. Saalmueller
Federal Research Institute for Virus Diseases of Animals
Paul Ehrlich Strasse 28
D - 72076 Tuebingen (Germany)
Tel: +49(0)7071/967256
Fax: /967303
Dr.R.M.Parkhouse
IAH Pirbright Laboratory
Ash Road, Pirbright, Working
GB - GU24 0NF (United Kingdom)
Tel: +44(0)1483/232441
FAX: /232448
Dr C.Stokes
Dept. of Veterinary Medicine
University of Bistol
School of Medicine
Langford, Bristol
GB - BS18 7DV (United Kingdom)
Tel: +44(0)1934/852581
Fax: /853145
Serotec Ltd
22 Bankside, Station Approach
Kidlington, Oxford, OX5 1JE
Tel +44 (0) 1865 852722
Fax +44 (0) 1865 373899
USA - Serotec Inc, Partners 1
1017 Main Campus Drive
Suite 2450, NCSU, Raleigh, NC27606
Tel: 1 800-265-7376
Fax: 919-515-9980
Good luck
Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator
_________________________________
Subject: MAbs for swine leucocytes
Author: Karine.Coutant@sandoz.com at INTERNET
Date: 22/09/97 21:45
Dear all,
Does anybody know about monoclonal antibodies against swine leucocytes
subsets:
-pan-leukocytes equivalents to human CD45
-T lymphocytes (CD3, CD4, CD8, CD25...)
-B lymphocytes (CD19, CD20 ...)
-Monocytes (CD14, CD11b,c,)
- and also, CD1a, CD34 ????
I thank you in advance for you help
Regards,
Karine Coutant