Re: Droplet charging

Gilbert McCrary (gmccrary@hiv.hjf.org)
Wed, 24 Sep 1997 17:34:53 -0400

We have sorted for years using a buffer which is 1% BSA in PBS and have not had
systematic problems with droplet charging. Occasionally we have had multiple
side-streams or a "fanning" effect in which a mist blows around the plates; but
these situations can generally be traced to dirty charge plates, faulty
connections, the wrong setting on our Elite's "front porch" or even a clog. I
don't know about the BD sorters but would be quite surprised if a 1% BSA buffer
could not be effectively used with them as well.

Happy sorting,

Gilbert

*****************************
Gilbert McCrary
Walter Reed Army Institute of Research
1600 E. Gude Drive
Rockville MD 20850
(301) 217-9410 ext. 1013
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Subject: Droplet charging
From: "Vincent Falco" <vincent.falco@es.nemc.org> at Internet_Gateway
Date: 9/23/97 6:46 PM

To All:
Would anyone suspect that a 1% BSA in a PBS buffer would create inappropriate
charging of droplets?Or any residual charging effects that would result in a
spurious drop trajectory?