Seeking position in Flow Cytometry &/or Confocal Microscopy.
Appropriate experience: ten years.
Qualifications: B.Sc. Biological Sciences 1978; Ph.D. Microbiology 1985.
Scientific training & experience:-
Research, application, development & tuition of Flow Cytometry
(FACStar Plus, FACSort, FACScan, Ortho Cytofluorograph 50-H,
Epics Elite, etc.) with on-line computer (H-P. 340/310, Ortho
2151, PC, Mac. etc.) data acquisition, presentation, off-line
transfer, analysis (Lysis-II, CellFit, Consort 30, WinList,
WinMDI, ModFit, MacCycle, etc.) & archiving (tape, M/O disk,
etc.); Bulk cell sterile flow-sorting & single cell
flow-cloning (ACDU); Many & varied techniques, especially
immunophenotyping (surface & cytoplasmic), DNA study
(cell-cycle, aopotosis, ploidy, etc.), et. al. & assorted
combinations; Establishment, supervision, management &
operation of sophisticated Flow Cytometry Core Facilities,
including consultation, liasion, scheduling, budgeting &
accounting, with direct involvement in many diverse research &
clinical projects; Confocal Laser Scanning Fluorescence
Microscopy (Bio-Rad MRC 1024, DVC 250, etc.) & 3-D imaging;
Field-testing of novel reagents & prototype instrument systems;
Computing (software & hardware, mainframe & micro-computers:
DOS/Windows & Mac.); Networking (FACSnet, WHPPC, Pegasus,
Banyan Vines, Pine, InterNet, W.W.W., H.T.M.L., etc.);
Microbiological & aseptic techniques, including continuous
chemostat culture & fermentation (design, instrumentation &
operation, etc.); Biochemical analyses; Lecturing to
undergraduate & medical students; Setting, marking & grading
term quizzes, final examinations & practical class reports;
Demonstrations for U.K. school A-level biology teachers'
refresher courses, undergraduate practical classes & student
tutorials; Help & supervision of instrument users, project
students & technicians; Scientific presentations at
international congresses, plus other symposia, seminars &
meetings; Production & co-ordination (including gathering of
material, preparation of sections, writing H.T.M.L. code, etc.)
for CD-ROMs designed to disseminate information & data to the
Cytometry community; Etc., etc., etc...
E-mail: Kruger@sorter.dfci.harvard.edu
'Phone: (617)-787-3196.
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