I am a microscopist and now starting the flow. My first experiments did not
work because I lost a majority of the cells during centrifugation. My
questions are:
1. Is it possible to use the indirect reaction or I should bind the FITC
directly to the primery antibody?
2. How can I optimize the centrifugation: time, g, volume, temperature.
3. Is there any good book about the flow for the beginner?
Thank you for any help. Dimitri
Dr. Dimitri Scholz | voice ++49 6032 705-403
Max-Planck-Institute | Fax ++49 6032 705-419
Dept. of Exp. Cardiology | Email dscholz@kerckhoff.mpg.de
Beneke-Str.2 |
D-61231 Bad Nauheim |
Germany |