Live cells stained with SYBR-14 (DNA stain, penetrates intact
membranes) fluoresces brighter (ab. 10x) than dead ones. Why does it happen?
Or, in the other words: what is the mechanism by which SYBR-14 binds to DNA?
Saying 'cells' I mean spermatozoa. I don't know what about other kinds
of cells.
Thanks in advance for any information.
Michal
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| National Research Institute of Animal Production
Michal Bochenek | Department of Animal Reproduction
mbochen@izoo.krakow.pl | 32-083 Balice / Krakow, Poland
| phone: 48 12 2856777 ext.145; fax: 48 12 2856733
| e-mail: zfr@izoo.krakow.pl
|
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