1) subscribe 2) help!

Jussi J. Saukkonen, Jr. , MD (jsaukkonen@bupula.bu.edu)
Thu, 02 Oct 1997 06:01:02 -0500

Hi,

I would welcome advice from any flow gurus out there. I have been
using PI, 7AAD, ethidium to look at viability of macrophages. after
releasing the cells from plastic with cold PBS or EDTA, I appear to have
30-40% nonviable cells. I have used non-tissue culture treated plates
as well as low adhesion clusters from Costar.
Any suggestions welcomed.

Thanks

Jussi Saukkonen MD
Pulmonary Center
Boston University