Re: Clumping of SV40 Transfected Cells

Shelton Murinda (sem124@psu.edu)
Tue, 30 Sep 1997 21:24:52 -0500

I am experiencing clumping of clumping of SV40 transfected human colon
cells after trypsinization prior to fixing with paraformaldehyde. This is
affecting apoptosis determinations. Does anyone have a quick fix?

SHELTON E. MURINDA
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Food Microbiology Laboratory
120A Borland Building
Department of Food Science
The Pennsylvania State University
University Park, PA 16802
Phone: 814-862-8475 (H); Phone: 814-863-2970 (W)
Fax: 814-863-6132

"The lizard that fell from the high iroko tree praised himself for having
jumped such a great height"
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