re: Mouse Monocyte Purification

Dennis Broud 301-827-5246 FAX 301-594-6289 (BROUDD@cder.fda.gov)
Tue, 30 Sep 1997 13:08:29 -0400 (EDT)

You might try a product called Lympholyte-M by Cedarlane Laboratoy of Hornby,
Canada. Phone number (416)878-8891.
It's available from Accurate Chemical and Scientific Corp in the US
at phone# 1-800-645-6264.
I'm not sure who has European distribution for it.

It is Nycograde Polysucrose 400 and Sodium Diatrizoate at a density of 1.0875
plus or minus 0.0010 at 25 degrees centigrade. Ph is 6.9
NYCOGRADE is a registered trade mark of Nycomed Pharma AS of Oslo, Norwary.

You need a different density of separation media for mouse cells than you do
for human cells as the mouse cells are somewhat smaller in size and denser.
Lympholyte-M is designed for mouse lymphocytes, I don't know exactly how many
monocytes you'll get with it, but it will be better than standard
Ficoll-Hypaque, I'm sure. Give Accurate's technical service people a call
maybe they can tell you what you can expect to get.

Cedarlane also makes a Lympholyte-rat with a differnt density for separating
rat lymphocytes cells as well.