Actually we try to stain dissociated epithelial cells and we have two kinds
of problem.
- First, staining of cells in suspension with antibody anti integrin
subunit seems very difiicult to detect and is not reproductible.
- Second, does anybody have some practice of VITAL staining of cells with
an intracellular cytoplasmic marker such as cytokeratin?
Does anybody have some experiment which would help us?
Thanks
PIERRE