On Tue, 30 Sep 1997, Tom Frey wrote:
> If you mean SYTO-14, see..
I mean SYBR-14, the component of sperm viability kit (Molecular Probes),
the second of the components is PI.
> ...if you have PI around the dead cells are going to show some quenching
>due to energy transfer.
Tom is right: SYBR14 used along with PI gives energy transfer (SYBR14->PI)
in the 'dead' PI positive cells.
But the other question exists. Using SYBR14 alone and with time as
a parameter I can see that after ab. 3-4 minutes of acquisition
fluorescence of cells (spermatozoa) divides into two 'branches' - the 1st
one remains on the same level while the 2nd one gradually drops down.
This phenomenon repeats in the succeeding loadings of the *same* sample.
What is a cause of ssuch behaviour?
Thanks in advance,
Michal
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| National Research Institute of Animal Production
Michal Bochenek | Department of Animal Reproduction
mbochen@izoo.krakow.pl | 32-083 Balice / Krakow, Poland
| phone: 48 12 2856777 ext.145; fax: 48 12 2856733
| e-mail: zfr@izoo.krakow.pl
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