this isn't a flow question, but you all seem to know where the antibodies
are! i've got a colleague who needs to show (by western blot) that his
nuclear fractions aren't contaminated with cytosolic proteins. i suggested
looking for ribsomes, ubiquitin or cytosolic beta-glucosidase or using a
fluid phase marker. do you have any suggestions as to what he can use, or
where to find the antibodies against these proteins?
thanks (in advance for this one and belatedly for the last.)
barbara
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Barbara A. Butcher, Ph.D.
James A. Baker Institute for Animal Health
Hungerford Hill Road
Cornell University
College of Veterinary Medicine
Ithaca, New York 14853
(607) 256-5647 Lab
(607) 256-5618 Office
(607) 256-5608 FAX
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