I hardly use any Dako antibodies so cant really comment there.
I do have a Medimachine. I bought it a while back for generating cell
suspensions from gastric biopsies. Other methods of mechanical disruption
seemed too dangerous to use with HIV infected samples. The machine is
small, fits nicely in the hood, well made but very expensive. I'd ask for a
demo for a week or two before you buy. For me it works OK, probably as good
as any mechanical method and is safe. Cell yields and viability ain't
great, enzymatic disruption gives much better yields for GALT tissue but
takes longer and can change some phenotypic markers.
Hope this helps
Simon Monard
Aaron Diamond Center
NYC